MLS Next Pro Eastern
| # | Team | PL | W | D | L | +/- | GD | PTS |
|---|---|---|---|---|---|---|---|---|
| 1 | Columbus Crew 2 | 13 | 10 | 1 | 2 | 30-12 | 18 | 31 |
| 2 | New England Revolution II | 12 | 7 | 3 | 2 | 15-13 | 2 | 25 |
| 3 | Rochester New York FC | 12 | 4 | 4 | 4 | 18-13 | 5 | 20 |
| 4 | New York City FC II | 12 | 5 | 3 | 4 | 26-17 | 9 | 19 |
| 5 | Philadelphia Union II | 11 | 4 | 3 | 4 | 14-15 | -1 | 17 |
| 6 | Toronto FC II | 12 | 5 | 1 | 6 | 22-25 | -3 | 16 |
| 7 | Inter Miami CF II | 13 | 5 | 1 | 7 | 17-30 | -13 | 16 |
| 8 | Orlando City B | 12 | 3 | 3 | 6 | 19-22 | -3 | 14 |
| 9 | Chicago Fire FC II | 12 | 2 | 4 | 6 | 17-26 | -9 | 11 |
| 10 | FC Cincinnati 2 | 12 | 3 | 0 | 9 | 15-27 | -12 | 9 |
MLS Next Pro Western
| # | Team | PL | W | D | L | +/- | GD | PTS |
|---|---|---|---|---|---|---|---|---|
| 1 | Tacoma Defiance | 13 | 7 | 4 | 2 | 35-15 | 20 | 28 |
| 2 | St. Louis City 2 | 12 | 8 | 2 | 2 | 25-14 | 11 | 27 |
| 3 | North Texas SC | 12 | 8 | 2 | 2 | 23-12 | 11 | 27 |
| 4 | Vancouver Whitecaps 2 | 14 | 6 | 5 | 3 | 28-18 | 10 | 25 |
| 5 | Houston Dynamo 2 | 12 | 7 | 1 | 4 | 18-13 | 5 | 22 |
| 6 | San Jose Earthquakes II | 13 | 6 | 2 | 5 | 23-19 | 4 | 21 |
| 7 | Minnesota United 2 | 11 | 5 | 3 | 3 | 23-14 | 9 | 21 |
| 8 | Sporting Kansas City II | 13 | 5 | 2 | 6 | 16-20 | -4 | 17 |
| 9 | Colorado Rapids 2 | 12 | 2 | 2 | 8 | 14-35 | -21 | 9 |
| 10 | Portland Timbers 2 | 11 | 1 | 2 | 8 | 10-26 | -16 | 7 |
| 11 | Real Monarchs SLC | 12 | 0 | 2 | 10 | 8-30 | -22 | 2 |